Sialic Acid as a Biomarker Studied in Breast Cancer Cell Lines In Vitro Using Fluorescent Molecularly Imprinted Polymers

Sialylations are post-translational modifications of proteins and lipids that play important roles in many cellular events, including cell-cell interactions, proliferation, migration. Tumor cells express high levels sialic acid (SA), which often associated with the increased invasive potential clinical tumors, correlating poor prognosis. To overcome lack natural SA-receptors, such as antibodies lectins enough specificity sensitivity, we have used molecularly imprinted polymers (MIPs), or “plastic antibodies”, nanoprobes. Because expression epithelial cell adhesion molecule (EpCAM) primary tumors is proliferation a more aggressive phenotype, EpCAM CD44 was initially analyzed. The SA-MIPs were for detection SA on surface breast cancer cells. Lectins specifically bind to a-2,3 a-2,6 variants analysis expression, both flow cytometry confocal microscopy. Here show correlation when using SA. We also demonstrate binding pattern lines Pre-incubation SA-derivatives inhibitors could reduce tumor cells, indicating SA-MIPs. In conclusion, may be new powerful tool diagnostic